Fig. 4

Inhibition of autophagy potentiated the anti-angiogenic properties of anlotinib. Tumor supernatant (serum free) was collected from Calu-1 cells after treatment with anlotinib and/or CQ 25 μM for 24 h. a to c, HUVECs were treated with corresponding tumor supernatant and subjected to a Transwell, b, scratch wound healing assay and c, tubular formation assay. d, The levels of VEGFA in the tumor supernatant were measured by ELISA. Data are represented as means ± SD of three independent experiments. n/s no significant, *P < 0.05, **P < 0.01. Scale bar: 100 μm