Fig. 5

Autophagy preserved angiogenesis through JAK2/STAT3/VEGFA pathway a, The expressions of p-JAK2, STAT3, p-STAT3, LC3-I/II and VEGFA were detected by western blotting after the lung cancer cells were treated with 500 nM RAPA for 24 h. b, Western blotting showed that S3I-201,100 μM dephosphorylates STAT3 and the elevated expression of VEGFA by RAPA was reversed by S3I-201. c, Calu-1 and A549 cells were pre-cultured with 3-MA 5 mM or CQ 25 μM for 2 h and then incubated with 20 μM anlotinib for 24 h. The expressions of p-JAK2, STAT3, p-STAT3 and VEGFA were tested by western blotting. *P < 0.05, **P < 0.01