Fig. 2

Hypoxia stimulation enhances internalization of macrophages-secreted exosomes by EOC cells. a Representative electron micrograph of exosomes isolated from macrophages-conditioned medium revealing the typical morphology and size (50–200 nm). Scale bar, 100 nm. b The diameter of exosomes (exo) derived from the conditioned medium of normoxic (N-exo) or hypoxic (H-exo) macrophages. c Western blot analysis showing the presence of CD63, CD81, CD9 and ALIX in the exosomes. d The concentration of exosomes was detected. e Representative confocal microscopy images showing the internalization of PKH67-labeled exosomes (green) derived from macrophages by SKOV3 cells under normoxia or hypoxia. Scale bar, 10 μm. *P < 0.05, **P < 0.01