Fig. 4

Exosomic miR-223 mediated cross-talk between macrophages and EOC cells. a Related miR-223 level in SKOV3 cells treated with PBS, N-Mcr-exo or H-Mcr-exo. b Fold change of miR-223 in SKOV3 cells cocultured with macrophages for the indicated time periods. c, d Fold change of miR-223 in SKOV3 cells cocultured with TAMs-conditioned, exosome-depleted TAMs-conditioned media or TAMs-conditioned media pretreated with GW4869 under normoxia (c) and hypoxia (d). e qRT-PCR for mature miR-223 in SKOV3 cells treated with RNA polymerase II inhibitor or PBS. f-k SKOV3 cells were treated with exosomes derived from the normoxic or hypoxic macrophages which were transfected with agomir or antagomir respectively, the related miR-223 level (f, g), cell apoptosis (h, i), the number of cell colonies (j), cell viability (k), IC50 for cDDP (l) was measured. *P < 0.05, **P < 0.01