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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Macrophages derived exosomes deliver miR-223 to epithelial ovarian cancer cells to elicit a chemoresistant phenotype

Fig. 5

Exosmic miR-223 inactivates PI3K/AKT pathway through PTEN targeting. a Detection of PTEN protein level (by immunoblotting) in EOC cells treated with PBS, N-Mcr-exo or H-Mcr-exo. b PTEN expression in SKOV3 cells cocultured with TAMs-conditioned media pretreated with DMSO or GW4869 under normoxia and hypoxia. c PTEN expression in SKOV3 cells cocultured with TAMs-conditioned, exosome-depleted TAMs-conditioned media under normoxia and hypoxia. d PTEN expression in SKOV3 cells treated with exosomes derived from the normoxic or hypoxic macrophages which were transfected with agomir or antagomir respectively. e Luciferase reporter assay in EOC cells cotransfected with wild-type (WT) or mutant (Mut) PTEN 3’UTR reporter gene and agomir or agomir-NC. f-i SKOV3 cells transfected with a plasmid expressing PTEN, then treated with exosomes derived from the normoxic or hypoxic macrophages which were transfected with agomir, PTEN protein level (f) cell apoptosis (g),cell viability (h), IC50 for cDDP (i) was measured. j PTEN, p-AKT and AKT protein levels in SKOV3 cells treated as indicated. *P < 0.05, **P < 0.01

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