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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Macrophages derived exosomes deliver miR-223 to epithelial ovarian cancer cells to elicit a chemoresistant phenotype

Fig. 6

Effects of exosomic miR-223 in cisplatin resistance in vivo. Exosomes derived from the normoxic or hypoxic macrophages which were transfected with agomir or antagomir respectively (N-Mcr-exo + agomir-NC, N-Mcr-exo + agomir, H-Mcr-exo + antagomir-NC, H-Mcr-exo + antagomir). SKOV3 cells were subcutaneously injected into BALB/c nude mice, we allowed potential tumors to grow for a week, then, cDDP (5 mg/kg) and exosomes (10 μg) or PBS were injected into the center of the xenograft tumors twice per week for 3 consecutive weeks (n = 6). a Growth curves of SKOV3 subcutaneous xenograft tumors. b Representative images of the excised tumors from the experiment on day 28 after tumor cell injection. c, d The changes of miR-223 (c) and PTEN (d) in tumor sections of mice treated as indicated above were plotted on day 28 after tumor cell injection. e Representative photographs of PTEN, p-AKT and AKT protein expression of tumors collected from each group detected by western blotting. f The immunohistochemistry analyses for PTEN and Ki67 staining were carried out on SKOV3 xenograft tumor sections. Representative staining were shown (× 200 magnification). g TUNEL analysis in tumors treated as indicated above (× 50 magnification). *P < 0.05, **P < 0.01

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