Fig. 10

EPB41L4A-AS2 functions as a tumor suppressor via the upregulation of RARRES1 expression. a Hierarchical clustering of the genes differentially expressed between the EPB41L4A-AS2 overexpression and control groups of UACC812 and BT549 cells. The red to blue color gradient indicates high to low expression levels, respectively. b GO analysis of the genes differentially expressed between the EPB41L4A-AS2 overexpression and control group. c KEGG analysis of the genes differentially expressed between the EPB41L4A-AS2 overexpression and control groups. The vertical axis represents the biological process or pathway category, and the horizontal axis represents the -log₂ (P value) of the significant biological process or pathway. d Validation of seven genes differentially expressed (RARRES1, ST18, BMP4, FOXA2, FOXL1, LRIG1 and RASSF1) in UACC812 and BT549 breast cancer cell lines via PCR array. e EPB41L4A-AS2 overexpression increased RARRES1 protein expression in UACC812 cells. f The knockdown of EPB41L4A-AS2 expression reduced RARRES1 protein expression in MDA-MB-453 cells. Data represent at least three independent experiments