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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: SHP1 and SHP2 inhibition enhances the pro-differentiative effect of phorbol esters: an alternative approach against acute myeloid leukemia

Fig. 3

Simultaneous downregulation of SHP1 and SHP2 additively enhances cell differentiation through SRC and β-catenin. a) Levels of SHP1 and SHP2 when HEL cells were individually or simultaneously silenced for both proteins. b) Expression levels of differentiation markers in HEL cells individually and simultaneously silenced for both PTPs. c) DNA content of HEL cells individually and simultaneously silenced for both PTPs. d) Levels of total and phosphorylated SRC protein in SHP1- and SHP2- silenced HEL cells. e) SRC protein levels in HEL cells upon RNAi-mediated downregulation with two different sequences. f) Induction of the expression of the surface markers after 48 h treatment with 20 nM PMA in SRC-silenced HEL cells. Comparisons were performed against control RNAi. g) Simultaneous downregulation of SHP1 and SRC or SHP2 and SRC in HEL cells. h) Induction of the expression of the surface markers after 48 h treatment with 20 nM PMA in HEL cells simultaneously downregulated for SRC and either SHP1 or SHP2. i) Levels of β-catenin in HEL cells individually and simultaneously downregulated for SHP1 and SHP2

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