Fig. 5

TRIM29 activates the Wnt/β-catenin signaling pathway in CRC cells. a The β-catenin protein expression and localization in modified SW620 and RKO cells was assessed by immunofluorescence. b The TRIM29 and β-catenin protein levels in the nucleus and cytoplasm of modified SW620 and RKO cells were assayed by Western blotting. LaminB1, β-actin, and α-Tubulin were used as internal controls. c TOP/FOP luciferase activity assay showed that β-catenin/TCF transcription activity was inhibited by knockdown of TRIM29, whereas it was activated by the overexpression of TRIM29. The data are presented as the mean ± SEM. from three independent experiments (***P < 0.001). d, e The mRNA and protein levels of the Wnt/β-catenin pathway target genes in modified SW620 and RKO cells were assayed by qTR-PCR and Western blotting. β-actin was used as an internal control. The data are presented as the mean ± SEM. from three independent experiments (***P < 0.001)