Fig. 2

PP242 exerts no effect on the subcellular localization of TFE3 fusion proteins. Renal carcinoma cell lines including HK2, UOK109 and UOK120 were incubated in the absence (Control) or presence of PP242 (4 μM) for 24 h. a, b Cells were stained with TFE3 (red) followed by immunofluorescence photomicrographic analysis. c, d The expression of TFE3 was determined by q-PCR and Western blot. GAPDH was used as an internal control. *P < 0.05, **P < 0.01. Scale bar, 50 μm