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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: TFE3 fusions escape from controlling of mTOR signaling pathway and accumulate in the nucleus promoting genes expression in Xp11.2 translocation renal cell carcinomas

Fig. 4

Binding between 14 and 3-3 proteins and TFE3 fusion proteins is reduced in the UOK109 and UOK120 cells. (a) 786-O cells were treated with PP242 (4 μM) or vehicle control (Con) for 24 h followed by immunoblot analysis of 14–3-3 proteins. GAPDH was used as an internal control. (b) HK-2 cells were treated with PP242 (4 μM) or vehicle control (Con) for 24 h followed by immunoblot analysis of 14–3-3 proteins. β-Tubulin was used as an internal reference. (c, d) UOK109 and UOK120 cells were treated with PP242 (4 μM) or vehicle control (Con) for 24 h followed by immunoblot analysis of 14–3-3 γ, 14–3-3 ζ, p-S6, S6 and mTOR proteins. (e, f) HK-2 cells were treated with Torin1 (4 nM) or vehicle control (Con) for 24 h followed by immunoblot analysis of 14–3-3 γ, phosphor-S6 (p-S6), S6 and mTOR proteins. (g) The binding between TFE3 and 14–3-3 γ was detected by co-immunoprecipitation (CO-IP). An irrelevant IgG was used as negative control

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