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Fig. 8 | Journal of Experimental & Clinical Cancer Research

Fig. 8

From: TFE3 fusions escape from controlling of mTOR signaling pathway and accumulate in the nucleus promoting genes expression in Xp11.2 translocation renal cell carcinomas

Fig. 8

TFE3 fusions could bind to the promoter of 4EBP3. (a) The transcriptional activity of TFE3 fusions with different splicing variants was detected with luciferase reporter assay in UOK109 and UOK120 cells. Cells were transfected with 4EBP3 promoter expressing plasmids (4EBP3 Promoter) or negative control plasmids (pGL4.0) for 48 h. (b) Western blot was used to determine the expression of TFE3 and 4EBP3 after 48 h transfection of TFE3 knockdown shRNA (Y3169 and Y3620), negative control shRNA (Y007) or blank control (Blank). (c) The interaction between TFE3 fusions and 4EBP3 promoter was detected by Chromatin immunoprecipitation (ChIP). PCR was applied to detect the target DNA fragments. (d) The predicted target binding sites to 4EBP3 were showed in the table. Data were presented as the mean ± SEM. *P < 0.05, **P < 0.01. The unpaired two-tailed Student’s t-test was used to calculate P value

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