Fig. 3
ZEB1-AS1 is upregulated in BLCA tissues and correlates with miR-200b and fascin-1 (FSCN1). (A and B) Kaplan–Meier analysis of overall (a) and disease free (b) survival based on ZEB1-AS1 expression levels in patients with BLCA from the TCGA database. Patients with BLCA were divided into ZEB1-AS1 high expression group (21% of the total, with the highest ZEB1-AS1 expression) and low expression group (21% of the total, with the lowest ZEB1-AS1). c ZEB1-AS1 expression levels were detected in 10 pairs of BLCA and adjacent normal mucosa tissue samples by qPCR. Data were analyzed by T-test. d The mRNA level of ZEB1-AS1 in 23 normal bladder tissues and 60 BLCA tissues was measured by qPCR. Data were analyzed by Mann-Whitney U test. e and f The correlation between ZEB1-AS1 and miR-200b (e), ZEB1-AS1 and FSCN1 (f) of 60 BLCA tissues. Data were analyzed by Spearman correlation analysis. g The mRNA level of ZEB1-AS1 in BLCA cell lines was measured by quantitative (qPCR). Data were analyzed by T-test. h Fluorescence in situ hybridization (FISH) assay in T24 and RT4 cells showing ZEB1-AS1 (red); nuclei were stained with 4′, 6-diamidino-2-phenylindole (DAPI; blue). i The mRNA level of ZEB1-AS1 in the nuclear and cytoplasmic fraction of T24 and RT4 cells by qPCR. Data are presented as mean ± standard deviation (SD). *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant