Fig. 5

ZEB1-AS1 inhibits apoptosis and promotes cell cycle and proliferation. a-b Apoptosis (a) and cell cycle (b) were detected by flow cytometry in T24 and RT4 cells after ZEB1-AS1 silencing or overexpression. Data were analyzed by T-test. c 5-Ethynyl-2′-deoxyuridine (EdU) assays on T24 and RT4 after ZEB1-AS1 silencing or overexpression. Data were analyzed by T-test. All images were taken at 200× magnification. d The proliferation of ZEB1-AS1-silenced or -overexpressing T24 and RT4 were detected by real time cell analysis (RTCA). Data were analyzed by T-test. e-h Xenograft model in nude mice. e Tumor growth was measured at the indicated days. f Nude mice at the end of the experiment. g Images of the excised tumors from each group. h Staining of Ki-67 and FSCN1 in the excised tumors. Data are presented as the mean ± standard deviation (SD). *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant