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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Long non-coding RNA ZEB1-AS1 regulates miR-200b/FSCN1 signaling and enhances migration and invasion induced by TGF-β1 in bladder cancer cells

Fig. 6

Transforming growth factor (TGF)-β1 inhibits miR-200b and promotes ZEB1-AS1 and fascin-1 (FSCN1) expression. a The mRNA level of ZEB1-AS1, miR-200b and FSCN1 in T24 and RT4 after treated with TGF-β1. Data were analyzed by T-test. b FSCN1 protein expression in T24 and RT4 cells treated with TGF-β1. Data were analyzed by T-test. c FSCN1 protein expression in T24 cells treated with TGF-β1 and transfected with siNC or siZEB1-AS1; untreated and not-transfected cells were used as control. Data were analyzed by T-test. d FSCN1 protein levels in T24 cells treated with TGF-β1 and transfected with miR-negative control (miR-NC) or miR-200b; untreated and not-transfected cells were used as control. Data were analyzed by T-test. e and f Transwell assays (without or with Matrigel) to detect migration (e) and invasion (f) of T24 and RT4 treated with TGF-β1 and transfected with siNC or siZEB1-AS1; untreated and not-transfected cells were used as control. g and h Transwell assays (without or with Matrigel) to detect the migration (g) and invasion (h) of T24 and RT4 treated with TGF-β1 and transfected with miR-NC or miR-200b untreated and not-transfected cells were used as control. Data were analyzed by T-test. i Hypothetical model illustrating the TGF-β1/ZEB1-AS1/miR-200b/FSCN1 axis. Data are presented as the mean ± standard deviation (SD). *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant

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