Fig. 3

Indo5 effectively inhibits c-Met and Trk coexpressing HCC cells growth in vitro and in vivo. (a) The expression profile of c-Met and Trk in the indicated cell lines. (b) HepG2 cells were transfected with either a scrambled control, c-Met siRNA, or TrkB siRNA. The expression levels of c-Met and TrkB were analyzed by real-time PCR analysis (upper panel) and Western blot (below panel). (c) HepG2 cells transfected with specific siRNA or control siRNA were treated with Indo5 (1 μM) for 48 h and cells growth was measured. The inhibition rate was calculated. (d) Indo5 inhibited the growth of HepG2 cell line tumor xenografts in nude mice (left panel). Images of tumors are shown in the right panel. The inhibition rate of Indo5 on HepG2 cell tumor xenograft growth was calculated according to the weight of tumors (e). (f) The effect of Indo5 on the growth of c-Met/TrkB co-expressing cell lines (MHCC97H, MHCC97L and A549) and other cells lines (SMMC-7721 and BEL-7402) (g) tumor xenografts in nude mice. The inhibition rates of Indo5 on the indicated cell line tumor xenografts were calculated (h)