Fig. 5
From: MicroRNA-137 reduces stemness features of pancreatic cancer cells by targeting KLF12
Suppression of KLF12 activity by miR-137 prevents Wnt/β-catenin signaling in pancreatic cancer cells. a KEGG pathway analysis showing that KLF12 expression was positively correlated with Wnt-activated gene signatures in the TCGA pancreatic cancer data set. b The indicated cells were transfected with TOP or FOP reporter and Renilla pRL-TK plasmids and subjected to dual-luciferase assays 48 h after transfection. The detected reporter activity was normalized to the Renilla activity. c RT–qPCR analysis of the expression of the established downstream targets for the Wnt/β-catenin pathway, including TCF4, TWIST, CMYC, MMP7, CCND1 and CD44 in the indicated cells d Western blot analyses the expression levels of AXIN1, APC, GSK-3B, β-catenin, phosphorylation β-catenin on Ser45 and nuclear β-catenin in the indicated cells e Subcellular β-catenin localization in the indicated cells was assessed by immunofluorescence staining