Table 1 The characteristics of the primers used for real-time PCR
From: MicroRNA-137 reduces stemness features of pancreatic cancer cells by targeting KLF12
GENE | Sequence (5′ - > 3′)Sequence (5′ - > 3′) | |
|---|---|---|
BMI-1 | Forward Primer | CCACCTGATGTGTGTGCTTTG |
Reverse Primer | TTCAGTAGTGGTCTGGTCTTGT | |
ABCG2 | Forward Primer | CAGGTGGAGGCAAATCTTCGT |
Reverse Primer | ACCCTGTTAATCCGTTCGTTTT | |
NANOG | Forward Primer | TTTGTGGGCCTGAAGAAAACT |
Reverse Primer | AGGGCTGTCCTGAATAAGCAG | |
SOX2 | Forward Primer | GCCGAGTGGAAACTTTTGTCG |
Reverse Primer | GGCAGCGTGTACTTATCCTTCT | |
OCT4A | Forward Primer | CTGGGTTGATCCTCGGACCT |
Reverse Primer | CCATCGGAGTTGCTCTCCA | |
LGR5 | Forward Primer | CTCCCAGGTCTGGTGTGTTG |
Reverse Primer | GAGGTCTAGGTAGGAGGTGAAG | |
KLF12 | Forward Primer | CGGCAGTCAGAGTCAAAACAG |
Reverse Primer | CGGCTTCCATATCGGGATAGT | |
TCF4 | Forward Primer | CAAGCACTGCCGACTACAATA |
Reverse Primer | CCAGGCTGATTCATCCCACTG | |
CCND1 | Forward Primer | GCTGCGAAGTGGAAACCATC |
Reverse Primer | CCTCCTTCTGCACACATTTGAA | |
MYC | Forward Primer | GGCTCCTGGCAAAAGGTCA |
Reverse Primer | CTGCGTAGTTGTGCTGATGT | |
TWIST1 | Forward Primer | GTCCGCAGTCTTACGAGGAG |
Reverse Primer | GCTTGAGGGTCTGAATCTTGCT | |
CD44 | Forward Primer | CTGCCGCTTTGCAGGTGTA |
Reverse Primer | CATTGTGGGCAAGGTGCTATT | |
MMP7 | Forward Primer | GAGTGAGCTACAGTGGGAACA |
| Â | Reverse Primer | CTATGACGCGGGAGTTTAACAT |
Control | UUAUUGCUUAAGAAUACGCGUAG | Â |
UCACAACCUCCUAGAAAGAGUAGA | Â | |
inhibitor | CTACGCGTATTCTTAAGCAATAA | Â |
miR-137 mimic | UUAUUGCUUAAGAAUACGCGUAG | Â |