Fig. 2

Lnc-PDZD7 suppresses the stemness of HCC cells. a, Expression of Lnc-PDZD7 was examined in Bel-7402, HepG2, SK-Hep-1, SNU-387 and SMMC-7721 cell lines by qRT-PCR. The data are shown as the means ± S.D. *Compared to Lnc-PDZD7 expression in LO2 (P < 0.05). b, Total RNA from the indicated cell lines was subjected to northern blot analysis to determine the molecular size and the expression level of Lnc-PDZD7. c, FISH was used to detect the endogenous Lnc-PDZD7 molecules (red) in Bel-7402 and HepG2. d-e, Representative images of sphere formation induced by sh-Lnc-PDZD7 or over-Lnc-PDZD7 transfection in HepG2 or Bel-7402, respectively. The surviving colonies were measured depending on their diameter. The data are shown as the mean ± SD of triplicate wells within the same experiment. *P < 0.05. f-g, Expression of CD133 and stemness-associated genes, including OCT4, NANOG and SOX2, was examined in siLnc-PDZD7 transfected HepG2 cells and over-Lnc-PDZD7 transfected Bel-7402 cells by Western blot. The data are shown as the means ± S.D. *P < 0.05