Fig. 5

The Lnc-PDZD7-EZH2-ATOH8 axis regulates stemness properties and chemosensitivity in HCC cells. a, The expression of ATOH8 was examined by qRT-PCR in Lnc-PDZD7-depleted HepG2 cells with EZH2 or EZH2-vec transfection. b, The expression of ATOH8 and CSC markers was examined by qRT-PCR in Lnc-PDZD7-depleted HepG2 cells with shATOH8 or shATOH8scr transfection. c, Sphere formation in Lnc-PDZD7-depleted HepG2 cells with shATOH8 or shATOH8scr transfection and EZH2-depleted HepG2 cells with shATOH8 or shATOH8scr transfection. d, Cell viability under different concentrations of 5-Fu treatment in Lnc-PDZD7-depleted HepG2 cells with shATOH8 or shATOH8scr transfection. e, The expression of ATOH8 and CSC markers was examined by qRT-PCR in EZH2-depleted HepG2 cells with shATOH8 or shATOH8scr transfection. f, Sphere formation in EZH2-depleted HepG2 cells with shATOH8 or shATOH8scr transfection. g, Cell viability under different concentrations of 5-Fu treatment in EZH2-depleted HepG2 cells with shATOH8 or shATOH8scr transfection. h, The expression of ATOH8 was examined by qRT-PCR in Lnc-PDZD7-overexpressing Bel-7402 cells with shEZH2 or shEZH2scr transfection. i, The expression of ATOH8 and CSC markers was examined by qRT-PCR in Lnc-PDZD7-overexpressing Bel-7402 cells with or without ATOH8. j, Sphere formation in Lnc-PDZD7-overexpressing Bel-7402 cells with or without ATOH8. k, Cell viability under different concentrations of sorafenib treatment in Lnc-PDZD7-overexpressing Bel-7402 cells with or without ATOH8. l, The expression of ATOH8 and CSC markers was examined by qRT-PCR in EZH2-overexpressing Bel-7402 cells with or without ATOH8. m, Sphere formation in EZH2-overexpressing Bel-7402 cells with or without ATOH8. n, Cell viability under different concentrations of sorafenib treatment in EZH2-overexpressing Bel-7402 cells with or without ATOH8 (n = 3, *P < 0.05)