Fig. 2
From: The anti-tumor growth effect of a novel agent DMAMCL in rhabdomyosarcoma in vitro and in vivo
DMAMCL induces RMS cell death via apoptosis in vitro. a RD, RH18, RH28, RH30 and RH41 cells were treated with different concentrations of DMAMCL for 48 h, harvested the cells with dead cells stained with propidium iodide. The percentage of cells in Sub-G1, G1 and S-G2–M phases is shown. b RD, RH18, RH28, RH30 and RH41 cells were treated with different concentrations of DMAMCL for 16 h, caspase-3/7 activity was evaluated. Each data point represents the mean, SD of triplicate wells. c RD, RH18, RH28, RH30 and RH41 cells were pretreated with Z-VAD-FMK (50 μM) for 2 h followed by the treatment of different concentrations of DMAMCL (RD for 10 μM, RH18 for 15 μM, and RH28, RH30 and RH41 for 7.5 μM). Cell survival was evaluated 48 h later by MTS. Each data point represents the mean, SD of triplicate wells