Fig. 5From: The anti-tumor growth effect of a novel agent DMAMCL in rhabdomyosarcoma in vitro and in vivoThe changes of Bim expression, p-NF-κB(p65) expression and ROS genenration after DMAMCL treatment in RMS cells. RD, RH18, RH30, and RH41 cells were treated with DMAMCL for 0, 4, 8, 12, 16, 24 h, total protein and total RNA was extracted for analysis of anti-Bim and anti-β-actin by western blot (a) and RT-qPCR (b). RMS xenografte-tumors were harvested at end points, total protein and total RNA was extracted for analysis of anti-Bim and anti-β-actin by western blot (c) and RT-qPCR (d). e RD and RH18 cells were treated with DMAMCL for 0, 4, 8, 12, 16, 24 h, total protein was extracted for analysis of anti-p-NF-κB(p65) and anti-β-actin by western blot. f RD and RH18 cells were treated with DMAMCL for 0, 4, 6 h, and the activity of ROS was detected. Bar, SD, *, P < 0.05Back to article page