Fig. 6
From: The anti-tumor growth effect of a novel agent DMAMCL in rhabdomyosarcoma in vitro and in vivo
Bim partially mediates the DMAMCL-induced cell death in RMS cells. RD cells were transfected with Bim-siRNAs and control-siRNA. 16 h later, the cells were treated with DMAMCL for 72 h. a RD cells were transfected with Bim-siRNAs and control-siRNA with or without DMAMCL by western blot. b Cell survival was detected by MTS assay. Each data point represents triplicate wells. c Cell survival was detected by MTS assay after normalized. Each data point represents triplicate wells. d Cell confluency(%) was calculated by Incucyte Zoom software by phase-contrast images from 0 to 72 h. Each data point represents triplicate wells. e Cell confluency(%) was calculated by Incucyte Zoom software by phase-contrast images from 0 to 72 h after normalized. Each data point represents triplicate wells. RH41 cells were transfected with Bim-siRNAs and control-siRNA. 16 h later, the cells were treated with DMAMCL for 72 h. f RH41 cells were transfected with Bim-siRNAs and control-siRNA with or without DMAMCL by western blot. g Cell survival was detected by MTS assay. Each data point represents triplicate wells. h Cell survival was detected by MTS assay after normalized. Each data point represents triplicate wells. i Cell confluency(%) was calculated by Incucyte Zoom software by phase-contrast images from 0 to 72 h. Each data point represents triplicate wells. j Cell confluency(%) was calculated by Incucyte Zoom software by phase-contrast images from 0 to 72 h after normalized. Each data point represents triplicate wells