Fig. 1
Immunohistochemical staining of WISP2 protein in ESCC tissues. a, Immunohistochemical staining images of WISP2 in esophageal normal mucosa (left panel), low-differentiated squamous cell carcinoma tissue and esophagitis tissue (middle panel), and high-differentiated squamous cell carcinoma tissues (right panel). b, RT-PCR was used to measure the WISP2 mRNA level in ESCC tissues and non-tumor tissues. N1: normal mucosa tissue 1; N2: normal mucosa tissue 2; T1: ESCC tissue 1; T2: ESCC tissue 2. c, Western blotting was used to measure the WISP2 protein level in ESCC tissues and non-tumor tissues. N1–3: normal mucosa tissue 1–3; T1-T3: ESCC tissue 1–3. d-e, The survival curves for WISP2 in ESCC patients with overall survival rate (d) and recurrence-free survival rate (e)