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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/2 and Akt pathways

Fig. 6

Anti-cancer efficiency of zoledronic acid against HeLa cells derived CSCs in vivo. Tumor tissues derived from HeLa cells derived CSCs bearing nude mice treated with different concentrations of zoledronic acid are exhibited (a). Tumor weights of HeLa cells derived CSCs bearing nude mice administered with different concentrations of zoledronic acid were examined on day 21 (b). Tumor volumes of HeLa cells derived CSCs bearing nude mice were detected every 3 days after treated with different concentrations of zoledronic acid until 21 days (c). Immunofluorescence staining and western blot analysis of stemness- and EMT-associated markers in tumor tissues derived from zoledronic acid-treated HeLa cells derived CSCs bearing nude mice (d-f). TUNEL staining of apoptotic cells in tumor tissues derived from zoledronic acid-treated HeLa cells derived CSCs bearing nude mice (g). The histogram shows the proportions of apoptotic cells (h). Western blot analysis of apoptosis and cell cycle arrest-associated proteins in tumor tissues derived from zoledronic acid-treated HeLa cells derived CSCs bearing nude mice (i-j). Scale bars represent 100 μM or 50 μM in inset. Control vs. 20, 40, and 80 μg/kg of zoledronic acid: * P < 0.05, ** P < 0.01, *** P < 0.001. Results are mean values ± SD of independent experiments performed in triplicate

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