Fig. 4

RBMS3 regulated Twist1 and MMP-2 expression. a, b In SUM-1315 and MDA-MB-231 cell lines, overexpression of RBMS3 inhibited the expression of Twist1, MMP-2. Western blot was used to detect the expression of Twist1, MMP-2 at the protein level. qRT-PCR was applied to examine the expression of Twist1, MMP-2 at the mRNA level. d, e Knockdown of RBMS3 contributed to the elevated expression of Twist1 and MMP-2. Similar methods were conducted as described in (a, b). c Overexpression of RBMS3 inhibited the expression of secreted MMP-2 protein in SUM-1315 and MDA-MB-231 cell lines. Western blot was used to detect the expression of MMP-2 in conditioned media. Data were shown as mean ± SEM, *P < 0.05. SCR = Scrambled control; Sh = Short hairpin; NC = Negative control