Fig. 6

RBMS3 inhibited Twist1-induced migration and metastasis in vitro and in vivo. a, b RBMS3-overexpressed and the control groups of SUM-1315 and MDA-MB-231 cells were transfected to overexpress Twist1, followed by western blots and qRT-PCR examination. c-h RBMS3 reversed the invasion and metastasis of Twist1 induced enhanced breast cancer cells in vitro and in vivo. (E-H) In SUM-1315 and MDA-MD-231 cells, transwell assay was used to detect invasion and migration ability. The lower panel of each picture showed the migrating and invading cell numbers. c, d Representative bioluminescence images showed the sizes and numbers of lung metastasis. The color scale indicated bioluminescence. Metastasis nodules plot was generated by the H&E-stained lung sections of nude mice (n = 9). Data were shown as mean ± SEM, *P < 0.05, **P<0.001 (Reviewer #1 comment 6)