Fig. 2

Depletion of miR-1271 inhibits cells invasion and EMT phenotype in Luminal A breast cancer. a The expression of miR-1271 in breast cancer cell lines as determined by RT-qPCR. b The expression of miR-1271in T47D and MCF7 cells transfected with miR-1271 inhibitor as determined by RT-qPCR. c Transwell invasion of cells as in (B). d and e, The mRNA (d) and protein (e) expression of EMT markers in cells as in (b) were detected by RT-qPCR and western blotting. f Immunofluorescence analyses of EMT markers in T47D cells. *P < 0.05. Scale bar, 50 μM