Fig. 4
Estrogen reverses TGF-β-induced EMT in a miR-1271 dependent manner. a The expression of miR-1271 in T47D cells after treatment with TGF-β1 at indicated concentration. b Transwell invasion assay of miR-1271-depleted T47D or control cells with TGF-β1 or/and E2 treatment. c Luciferase reporter analysis of TGF-β signaling activity in cells as in (B). d Localization of SMAD2 in cells as in (B) as determined by immunofluorescence staining. e The expression of pSMAD2 in cells as in (A) by western blotting. f The expression of SNAI1/2, TWIST1 and ZEB1 in cells as in (A) as determined by RT-qPCR. *P < 0.05. Scale bar, 50 μM