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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Estrogen receptor-α-miR-1271-SNAI2 feedback loop regulates transforming growth factor-β-induced breast cancer progression

Fig. 5

miR-1271 inhibits cell invasion and EMT by targeting SNAI2. a The predicted binding of miR-1271 with SNAI2 3′-UTR. b Dual luciferase reporter assay was performed to validate the miR-1271 target, SNAI2. c The expression of miR-1271 in indicated cells as determined by RT-qPCR. d The expression of SNAI2 in indicated cells as determined by western blotting. e Transwell invasion assay of miR-1271-overexpression MDA-MB-231 transfected with SNAI2 plasmid, as well as in control cells. f and g The mRNA (F) and protein (G) expression of EMT markers in cells as in (e) were detected by RT-qPCR and western blotting. h Immunofluorescence analyses of EMT markers in cells as in (e). *P < 0.05. Scale bar, 50 μM

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