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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Overexpression of RNF38 facilitates TGF-β signaling by Ubiquitinating and degrading AHNAK in hepatocellular carcinoma

Fig. 2

RNF38 involved in tumor motility and invasiveness, apoptosis and proliferation in vitro and vivo. a. Western blot detected RNF38 expression in four HCC cell lines (PLC/PRF/5, HCCLM3, HepG2, Huh7). b. The efficiency of transfection in HCCLM3 and HepG2 cell lines were confirmed by qRT-PCR and western blot. c. Cell proliferation was measured by the CCK-8 assay. d. Cell migration in HCC cells with different RNF38 expression was assessed by wound healing assay. e. Cell invasion ability in HCC cells was assessed by transwell assay. f. HCC cells apoptosis rate was evaluated by flow cytometry. g. Tumor derived from nude mice implanted with the HCCLM3-NC, HCCLM3-shRNA2, HepG2-vector, HepG2-RNF38 cell lines (n = 5). h. Tumor growth curves of the subcutaneous xenografts. I. The representative photos showed lung metastasis. Scale bar: 100 um.* p < 0.05, ** p < 0.01, *** p < 0.001

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