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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Overexpression of RNF38 facilitates TGF-β signaling by Ubiquitinating and degrading AHNAK in hepatocellular carcinoma

Fig. 3

RNF38 induced HCC cell EMT. a. The cellular morphology of four cell lines (HCCLM3-NC, HCCLM3-shRNA2, HepG2-vector, and HepG2-RNF38) was detected by phase contrast microscope. b. Expression of epithelial and mesenchymal markers was compared among HCCLM3-NC, HCCLM3-shRNA2, HepG2-vector, and HepG2-RNF388 cell lines. c. Semi-quantitative analysis of western bolt. d. Representative immunofluorescent images of RNF38, E-cadherin, Vimentin, and β-catenin in HCCLM3-NC, HCCLM3-shRNA2, HepG2-vector, and HepG2-RNF38 cells. e. Serial section and IHC staining showed expression of RNF38, E-cadherin, Vimenail. f. Xenograft tumor tissues IHC staining showed expression of RNF38, E-cadherin, Vimentin, and Snail. Scale bars 50 μm. The typical EMT cells are represented as black arrows. * p < 0.05,** p < 0.01, *** p < 0.001

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