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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Overexpression of RNF38 facilitates TGF-β signaling by Ubiquitinating and degrading AHNAK in hepatocellular carcinoma

Fig. 5

High level of RNF38 faciliates TGF-β pathway by ubiquitinating and degrading AHNAK. a. Western blot showed the phosphorylation level of ERK1/2, AKT, Smad2/3 when the RNF38 was knockdown or overexpression. b. Western blot showed phosphorylation level of Smad2/3 in HCCLM3-NC, HCCLM3-shRNA2, HepG2-vector and HepG2-RNF38 cells treated by (TGF-β, 5um) for 0 h, 2 h, 4 h, 8 h. c. The levels of main EMT molecules and p-Smad after TGF-β and/or inhibitor incubation. d. The levels of main EMT molecules and p-Smad in HCCLM3 co-transfected NC, shRNF38, siAHNAK. e. Cell migration and invasion of HCCLM3 was inhibited by shRNF38, which was further rescued by AHNAK suppression. f. The cell EMT was inhibited via knockdown RNF38, and rescued by the consequential downregulation of AHNAK in HCCLM3 cells. The shape of cancer cells and EMT markers were showed. Scale bars 100 um

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