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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: RETRACTED ARTICLE: A positive feedback loop consisting of C12orf59/NF-κB/CDH11 promotes gastric cancer invasion and metastasis

Fig. 3

Expression level of C12orf59 influence GC EMT and angiogenesis. a Western blotting reveals that knockdown of C12orf59 by shRNAs resulted in an increased expression of E-cadherin and α-catenin and a decreased expression of fibronectin and vimentin in both MKN-45 and AGS cells, compared with that in shcontrol treated cells. Decreased levels of E-cadherin and α-catenin and increased levels of fibronectin and vimentin were examined in HGC-27/C12orf59 cells compared with that in control HGC-27/vector cells. b IF staining shows a downregulated expression of E-cadherin and an upregulated expression of vimentin in HGC-27/C12orf59 cells compared with that in control HGC-27/vector cells with a corresponding change in the number of positive staining cells. c and d. The abilities of in vitro capillary tube formation (c) and migration (d) of HUVEC were significantly decreased after incubating with culture medium of C12orf59-silenced GC cell, while potentially strengthened after incubating with culture medium of C12orf59-transfected GC cell

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