Fig. 1

CRIP1 is overexpressed in both CRC tissues and cell lines. a. Left panel: Western blot assay for CRIP1 expression in ten CRC tissues (T) and matched normal adjacent normal tissues (N) from the same patient. Right panel: Quantification of CRIP1 expression in CRC tissues and adjacent normal tissues of ten patients. b. Left panel: IHC staining indicated that CRIP1 protein expression was increased in human CRC compared with normal intestinal epithelium (four representative slides was shown). Right panel: Graphical illustration of statistical CRIP1 distribution in CRC tissues. CRIP1 is significantly highly expressed in CRC tissues compared with that in adjacent non-tumorous tissue. c. Real-time PCR analyzed the expression of CRIP1 in NCM460 and six different CRC cell lines. The values represented relative CRIP1 level after normalization to the expression of β-tubulin (means ±SD, N = 3) d. Western blot analysis for the expression of CRIP1 in NCM460 and CRC cell lines. Immunosignals were quantified by densitometric scanning