Fig. 3

Knockdown of CRIP1 further suppresses 5-FU suppressed CRC cell proliferation both in vitro and in vivo. a. Western blot assay was used to detect the silencing of CRIP1 in HCT116 and SW480 cells. b. CCK8 assay was used to study the effects of siCRIP1 on 5-FU inhibited CRC cell proliferation. c. Annexin V-FITC/PI flow cytometry assay showed the effects of siCRIP1 on 5-FU induced apoptosis. Bars on the right panel represent percentage of cells in Q2 + Q4. d. TUNEL assay showed the effects of siCRIP1 on 5-FU induced apoptosis of CRC cells. Bars on the right panel represent the percentage of TUNEL positive cells. e. IHC staining showed the expression of CRIP1 in subcutaneous tumors formed by CRIP1 stable silencing CRC cells. Right panel represents the growth pattern of subcutaneous tumors