Fig. 5

miR-96-5p targets PTEN and affects PI3K activation. a PTEN distribution in tumoral samples with altered PTEN (mutated or deleted), with not altered PTEN and in normal samples. b PTEN distribution in tumoral samples with altered PTEN or with down-regulation of PTEN higher than 0.5 log2 folds and in normal samples. c Position of the miR-96-5p target site in the 3’UTR of PTEN predicted by miRwalk 3.0. d Luciferase activity of PTEN-3’UTR-WT reporter gene in H1299 cell line. Cells were transiently transfected with miR-96-5p mimic or control mimic. The histogram bars show the means of three experiments performed in duplicate, **P < 0.001. e Luciferase activity of PTEN-CDS-WT reporter gene in H1299 cell line which were transiently transfected with miR-96-5p mimic or control mimic. Bars show the means of two experiments performed in triplicate. f RT-qPCR graph showing miR-96-5p expression level in Cal 27 cell line after miR-96-5p inhibitor transfection, **P < 0.001. g Western-blot analysis of PTEN, p-AKT and AKT protein expression level in Cal 27 cells that were transfected with miR-96-5p inhibitor or control inhibitor. NC: Negative control, T: tumor