Fig. 2

NAP1 promotes NSCLC cell invasion. (a, b) The expression levels of NAP1 in two NSCLC cell lines determined by qRT-PCR (a) and Western blotting (b). (c) Overexpression of NAP1 in H460 cells determined by qRT-PCR. (d) The effect of NAP1 overexpression on cell invasion determined by Transwell. (e) The effect of NAP1 overexpression on secretion of MMP2 and MMP9 in H460 cells determined by ELISA. (f) Knockdown of NAP1 in H661 cells determined by qRT-PCR. (g) The effect of NAP1 knockdown on cell invasion determined by Transwell. (h) The effect of NAP1 knockdown on MMP9 secretion in H661 cells determined by ELISA. EV: empty vector; NAP1 O/E: NAP1 overexpressing vector; shCONT: non-targeting shRNA; shNAP1–1 and shNAP1–2: two shRNAs against the NAP1 gene. **p < 0.01