Fig. 6

NAP1 is a new HSP90-interacting partner and required for its mediated Vimentin in NSCLC cells. (a and b) The interaction between HSP90 and NAP1 proteins determined by IP. The immunoprecipitates from NAP1 overexpressing H460 cells (a) and H661 cells (b) were pulled down with anti-Flag and anti-NAP1 antibodies, respectively, followed by Western blotting with antibodies against HSP90 and NAP1. Preimmune IgG was used as a control. (c) The effect of 17-AAG on the HSP90-NAP1 interaction. (d) The effect of NAP1 knockdown on molecules related to EMT. (e and f) The effect of 17-AAG (e) and HSP90 knockdown (f) on Vimentin protein levels. (g) The effect of NAP1 knockdown on Vimentin protein levels in HSP90 overexpressing and control cells. In (c-g), H661 cells were used as a cell model. EV: empty vector; HSP90 O/E: the HSP90 gene overexpressing vector