Fig. 1

The expression of GAGE7B in gastric cancer tissues and its correlation with clinicopathological features. a and b. Microarray data showed 700 downregualted genes and 450 upregualted genes (fold-change ≧2, P <  0.05) between metastatic cancer tissues (M group) and nonmetastatic cancer tissues (N group) (a). The differentially expressed genes were further identified according to the fold-change (≧4), raw value (≧300) and P value (P < 0.05). The results showed that 13 genes were significantly downregulated and 13 genes including GAGE7B were significantly upregulated in metastatic tissues (b). c-e. The result of RT-qPCR confirmed that the expressions of PHGR1 and GAGE7B, but not ZNF503, were upregulated in metastatic cancer tissues, compared with nonmetastatic cancer tisssues (t-test, P = 0.838, P = 0.027, P = 0.038, respectively). f. GAGE7B mRNA expression was significantly increased in poorly differentiated gastric cancer cell lines BGC823, MKN45 and SGC7901, compared with the well differentiated cell line AGS (t-test, P = 0.007, P = 0.008 and P = 0.006, respectively). g. The protein expression of GAGE7B was demonstrated to be negative in nontumorous tissues. In contrast, positive staining of GAGE7B protein was observed in tumor samples and GAGE7B protein expression was increased in metastatic gastric cancer tissues in comparison with nonmetastatic cancer tissues (× 200). The GAGE7B expression was further increased in metastatic loci, compared with the primary lesions. h and i. In Kaplan-Meier survival analysis and Log-rank Test, the patients with a higher GAGE7B expression had shorter OS (e, P = 0.020) and DFS than those with lower expression (f, P = 0.008)