Fig. 2

Ex vivo expanded DNT cells inhibit late stage tumor growth in xenograft models. NSG mice were inoculated subcutaneously with NCI-H460 (a, b, d) or XDC137 (c and e) in 50% Matrigel solution and grown to ~100mm³. After tumors were established, tumor bearing mice were randomized into groups and treated with peritumoral injection of IL-2 with or without DNT cells on day 0, 3 and 6. (a and c) Tumor volume was measured at indicated time points. Arrows indicate DNT cell treatments. Results represent one of three independent experiments, each consisting of 5 mice per treatment group (a), or one experiment consisting of 3 mice per treatment group (c). b Survival of mice receiving IL-2 (control) or IL-2 + DNT cells (DNT) d and e. Immunohistochemistry staining with anti-human CD3 antibody on resected tumor xenografts. Representative sections of CD3⁺ DNT cells within tumor xenograft from both groups are shown at 21 days for NCI-H460 xenografts (d) and at 71 days for XDC137 xenografts (e) Quantified CD3⁺ staining density of whole xenograft sections, as determined by digital analysis of positive stain per area analyzed. Each dot represents one mouse and horizontal bars represent the mean ± SEM. Data shown are representative of 2 separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001, by two-tailed unpaired t-test (a, c, d and e) or log-rank (b)