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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Targeting late-stage non-small cell lung cancer with a combination of DNT cellular therapy and PD-1 checkpoint blockade

Fig. 5

Anti-PD-1 antibody enhances infiltration of cytotoxic DNT cells into tumor xenografts. Tumor-bearing NSG mice received peritumoral injection of DNT cells with or without anti-PD1 treatment. A. Representative flow cytometric analysis of DNT cells pre-infusion and tumor infiltrating DNT cells 21 days post infusion. The data shown represent results from 2 independent experiments. b Immunohistochemistry analysis of DNT cells. Nine days post DNT cell infusion, tumor xenografts were harvested and stained with anti-human CD3 antibody and quantified by Aperio Image-scope. Representative staining and analysis of tumor infiltrating DNT cells in indicated treatment groups are shown. Each dot represents one mouse and horizontal bars represent the mean ± SEM. Data shown are representative of 2 separate experiments. c-e Flow cytometry analysis of tumor infiltrating DNT cells. Frequency of NKG2D+ or DNAM-1+ DNT cells (c). IFNγ+ and TNFα+ DNT cells (d), perforin+, granzyme B+ and CD107a+ DNT cells (e). Representative results shown as mean ± SEM from 3 tumors of 2 separate experiments are shown. (*p < 0.05 by two-tailed unpaired t-test)

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