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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Angiotensin II promotes ovarian cancer spheroid formation and metastasis by upregulation of lipid desaturation and suppression of endoplasmic reticulum stress

Fig. 4

ANGII and AGTR1 formed a positive feedback loop that enhanced ovarian cancer progression. a Secretion of ANGII by ovarian cancer cells (Ovca429 and HM) into the culture medium. The ANGII level was measured by enzyme immunoassay. b Accumulation of ANGII in the culture medium of Ovca429 cell was measured. c & d AGTR1 and AGT mRNA expression after ANGII treatment, which ranged from 0 to 30 nM and was measured by RT-qPCR after ANGII treatment. (e) ANGII levels in ascites of xenografted mice (each group n = 4). Sham: mice injected with Hanks’ buffer without cancer cells; CTR: mice inoculated with cancer cells without drug administration; ANGII: mice inoculated with cancer cells and treated with ANGII (100 nM) at days 1, 3, and 5; ascites was collected at day 30. Losartan: mice inoculated with cancer cells and treated with losartan (10 μM), ANGII+losartan: mice inoculated with cancer cells and treated with ANGII (100 nM) and losartan (10 μM) (f). ANGII levels in ascites of ovarian cancer patients (n = 5) and choledocholithiasis patients (n = 6). The data are presented as means ± SEM from at least three experiments; * p < 0.05, ** p < 0.01, *** p < 0.001 against choledocholithiasis patients

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