Fig. 5

ANGII enhanced lipid metabolism and promoted MCS formation. a Based on proteomic analysis, differentially expressed proteins after ANGII treatment are presented by heatmap. b GO enrichment analysis was employed to analyze the biological function of the upregulated proteins. c GO enrichment analysis was employed to analyze the biological function of the downregulated proteins. d and e Changes in SCD1 and EHHADH protein levels after treatment with ANGII (30 nM) confirmed by western blotting. f The expression of SCD1 and SREBP1in HM cells after ANGII treatment measured by western blotting. g The expression of SCD1 and SREBP1 after ANGII treatment in Ovca429 cell were measured by western blotting. h Western blotting confirmed ANGII induced-SCD1 expression was suppressed by blocking of MEK/ERK1/2 and PI3K/AKT pathways. The data are presented as means ± SEM from at least 3 experiments and significant difference are indicated (* p < 0.05, *** p < 0.001 against control)