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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Angiotensin II promotes ovarian cancer spheroid formation and metastasis by upregulation of lipid desaturation and suppression of endoplasmic reticulum stress

Fig. 6

ANGII induced SCD1 expression resulted in alleviation of ER stress in MCS. a SCD1 inhibitor was employed to block the ANGII effect on spheroid formation. The data are presented as means ± SEM from at least three experiments with significant differences against control indicated (HM cells, ** p < 0.01,*** p < 0.001). b shRNA vector was used to silence the SCD1 expression and verify the involvement of SCD1 in the ANGII induced spheroid formation. The data are presented as means ± SEM from at least three experiments with significant differences against control indicated (HM cells, * p < 0.05, ** p < 0.01). c Necrotic marker, BiP, CHOP, and p-PERK expression in MCS was increased during MCS formation, but suppressed by ANGII treatment and reversed by the SCD1 inhibitor. Protein levels of BiP, CHOP, and p-PERK after various treatments, quantified by western blot. d SCD1 inhibitor (20 μg/kg) was used to block the ANGII effect on tumor formation and cancer metastasis in xenografted mice. Tumor nodes and tumor weights were and measured. The data are presented as means ± SEM (n = 3 for each group, * p < 0.05 against the corresponding control group). e SCD1 mRNA expression levels in AGTR1 high expression patients and AGTR1 low expression patients. AGTR1 expression levels belonging to the lower tertile were defined as the “AGTR1 low” group and the upper tertile defined as the “AGTR1 high” group. f Survival expectation of patients with different expression patterns of AGTR1 and SCD1 were assessed by Kaplan-Meier analysis. Logrank analysis was performed to compare the difference between AGTR1-Low/SCD1-Low group and the AGTR1-High/SCD1-High group. g Schematic diagram summarizing the molecular mechanism for ANGII/AGTR1 enhanced the ovarian cancer MCS formation, MCS growth, and metastasis. Arrows in red: the positive feedback loop of ANGII/AGTR1 in ovarian cancer cells. Arrows in green: the classic Gq dependent signaling pathways. Arrows in purple: the transactivation of EGFR by AGTR1. Arrows in blue: the SREBP pathways increase lipogenesis and suppress ER stress, enhancing MCS formation and metastasis

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