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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Long noncoding RNA EPB41L4A-AS2 inhibits hepatocellular carcinoma development by sponging miR-301a-5p and targeting FOXL1

Fig. 4

MiR-301a-5p was determined as a direct target of EPB41L4A-AS2. a-c FISH and qRT-PCR results demonstrated that EPB41L4A-AS2 was mainly expressed in the cytoplasm of SMMC-7721 and QGY-7703 cells; d-e. The expression of EPB41L4A-AS2 and miR-301a-5p is negatively correlated in HCC cells; f The direct binding sites between miR-301a-5p and EPB41L4A-AS2; g Luciferase reporter assay for confirmation of direct binding relationship between miR-301a-5p and EPB41L4A-AS2 was performed with luciferase reporter plasmids of wild type EPB41L4A-AS2 and mutant EPB41L4A-AS2. h The association between EPB41L4A-AS2, miR-301a-5p and Ago2 was ascertained by analyzing cell lysates using RNA immunoprecipitation with Ago2 antibody. Real-time PCR was applied to detect the change in EPB41L4A-AS2 expression level in the substrate of RIP assay in miR-301a-5p-overexpressed HCC cells

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