Fig. 2

Correlation between CIC-TEX and CIC-TEX-induced changes in mRNA and miRNA profiles. a Numbers of ≥2-fold enriched mRNA (signal strength ≥1000) in CIC-TEX-treated v6kd and Tsp8kd cells sorted according to ≥2-fold higher versus comparable or lower recovery in CIC-TEX than kd cells; b numbers of miRNA ≥1.5-fold upregulated in CIC-TEX-treated v6kd and Tsp8kd cells sorted according to ≥1.5-fold higher versus comparable or lower recovery in CIC-TEX than kd cells; c Correlation between ≥1.5-fold up- or downregulated mRNA in CIC-TEX treated v6kd and Tsp8kd cells sorted according to reverse miRNA recovery in CIC-TEX-treated cells; d major activities (IPA-based Reactome analysis) of ≥2-fold up- or downregulated mRNA that are predicted targets of inversely recovered miRNA (miRNA database, target scan database) in both CIC-TEX-treated v6kd and Tsp8kd cell (List of synonyms: Additional file 1: Table S1). mRNA and miRNA recovery are more strongly affected by CIC-TEX treatment in CD44v6kd than Tsp8kd cells. However, at the mRNA and the miRNA level coculture-induced changes rarely correlate with the CIC-TEX content. Instead, there is a strong correlation between coculture-induced up- or downregulated miRNA and reversely down- or upregulated mRNA in CIC-TEX-treated kd cells, indicating that not the CIC-TEX content, but CIC-TEX-induced target cell activation is dominating