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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Pancreatic cancer-initiating cell exosome message transfer into noncancer-initiating cells: the importance of CD44v6 in reprogramming

Fig. 3

CIC-TEX-initiated changes in RTK and downstream signaling molecules in CD44v6kd and Tspan8kd cells. a Signaling array of A818.4-v6kd cells cultured for 72 h with/without CIC-TEX. The relative signal strength was evaluated by ImageJ; significant differences by coculture with CIC-TEX: *. Flow-cytometry and WB analysis of (b,c) RTK expression in kd-TEX- or CIC-TEX-treated kd cells and (d,e) major pathway-engaged cytosolic signaling molecules; b,d mean % stained cells±SD (3 assays), significant differences by coculture with TEX: *; c,e representative examples and relative signal strength±SD of 3 independent experiments including p-values for kd cells compared to CIC-TEX-treated kd cells; f pathways from miRNA to RTK (IPA-based STRING analysis after predicted target mRNA selection by microrna.org and targetscan.org) for ≥2-fold upregulated miRNA (framed) and ≥ 2-fold reduced mRNA recovery in CIC-TEX-treated v6kd or Tsp8kd cells compared to untreated kd cells; g IPA-based STRING analysis after predicted target mRNA selection by microrna.org and targetscan.org for ≥2-fold reduced miRNA in CIC-TEX-treated compared to untreated v6kd cells and of ≥2-fold upregulated predicted mRNA targets that are engaged in signal transduction. h Flow-cytometry analysis of ex vivo harvested intrapancreatic A818.4-v6kd cells from nude mice with/without weekly iv CIC-TEX treatment; mean % stained cells±SD (3 tumors), significant differences by CIC-TEX treatment: s; i Representative immunohistology examples of A818.4-v6kd and -Tsp8kd shock-frozen tumor sections with/without CIC-TEX treatment stained with the indicated antibodies (scale bar: 100 μm). (List of synonyms: Additional file 1: Table S1). CIC-TEX treatment strongly affects RTK expression and downstream signaling molecules in vitro and in vivo. Changes in the recovery of mRNA engaged in signal transduction (Additional file 1: Figure S2d, S2e) are accompanied at a noteworthy frequency by reversely altered miRNA expression in CIC-TEX-treated kd, predominantly v6kd cells

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