Fig. 4

CIC-TEX-promoted apoptosis-resistance in CD44v6kd and/or Tspan8kd cells. a Flow-cytometry of cells cultured for 48 h in the presence of cisplatin; mean % AnnexinV+ and AnnexinV+/PI+ cells±SD (triplicates); significant differences of wt cells versus CIC or kd cells: *; significant differences by CIC-TEX treatment: s. b mRNA that expression differed by ≥2-fold after CIC-TEX treatment in Tsp8kd (blue) or v6kd (violet) cells were sorted by KEGG analysis according to the engagement in distinct apoptotic processes. c-f Flow-cytometry analysis of apoptosis-related receptor and cytoplasmic signaling molecules in A818.4, −v6kd and CIC-enriched cells and in v6kd cells cocultured with v6kd- or CIC-TEX; mean % stained cells±SD (3 assays), significant differences between wt cells, v6kd cells and CIC: *, significant differences by coculture of v6kd cells with TEX: s. g Representative immunohistology examples of A818.4 and -v6kd shock-frozen tumor sections from untreated or CIC-TEX-treated mice stained with the indicated antibodies (scale bar: 100 μm). (List of synonyms: Additional file 1: Table S1). Slightly reduced apoptosis resistance of v6kd and Tsp8kd tumor cells becomes mitigated by CIC-TEX treatment. The impact of CIC-TEX on apoptosis resistance is mostly restricted to the regulation of molecules engaged in the intrinsic pathway of apoptosis induction. An exception is the increased expression of drug transporters in CIC-TEX-treated v6kd cells