Fig. 1

ZL170 targets the TGFβ and BMP signaling pathways in MDA-MB-231 cells. a Chemical structure of ZL170 that was extracted from the dry whole bodies of P. americana. b-d RNA sequencing analysis of vehicle- and ZL170-treated MDA-MB-231 cells. Pie chart denotes the distribution of total transcripts changed in ZL170-treated cells as compared with vehicle-treated cells (b). Heat map of all targets in vehicle- and ZL170-treated cells (c). Gene set enrichment analysis (GSEA) of expression of signature genes involved in multiple cellular functions (d). e qPCR analysis validated TGFβ/BMP-targeted genes as well as genes encoding TGFβ superfamily receptors and ligands. Data are represented as mean ± S.D. (n = 3 independent experiments). * P < 0.05, ** P < 0.01, two-sided Student’s t-test